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Virus Purification
The optimal kit for construct screening
Vivapure AdenoPACK 20 is the small scale kit in the AdenoPACK series, purifying up to 1 x 1012 viral particles from 20 ml cell culture. Especially when testing new constructs, parallel and fast purifications of different adenoviruses are essential. This kit allows the rapid, simple and affordable spin column based purification of 6 different samples in parallel and bridges a gap in the CsCl density gradient method – for the first time adenovirus can efficiently be purified from less than 100 ml cell culture volume!
Fast purification of up to1x1013 viral particles
Vivapure AdenoPACK 100 is optimally suited for adenovirus purification from up to 200 ml cell culture for in vitro transfection. This flexible kit contains two AdenoPACK 100 units, which can be either used in tandem for the purification of up to 200 ml cell culture supernatant or individually for purifying virus from up to 60 ml cell culture. The purification is pursued manually with a syringe optimally attached to a retort stand. However, for even more convenience, protocols are provided for optionally running the virus purification with a peristaltic pump or with an infusion pump, in additional to detailed instructions for a manual operation supplied with the Kit. The accessories needed for the operation with a pump are supplied as individual products.
Kit for adenovirus purification and concentration
Vivapure AdenoPACK 500 is the direct upscale kit to the AdenoPACK 100, for adenovirus purification. In only 2 hours up to 3 x 1013 adenovirus particles are purified and concentrated from 500 ml cell culture. This completely ready-to-use kit is conveniently operated by a laboratory pump, offering optimal flow control and minimal hands-on time. This easy to use product replaces lengthy and inefficient cesium chloride density gradient methods.
Fast purification of up to 8 x 108 viral particles
Vivapure LentiSELECT 40 is optimally suited for lentivirus purification from up to 40 ml cell culture and contains all components necessary for 4 purifications. Up to 8 x 108 viral particles are recovered in less than one hour. In contrast to traditional ultracentrifugation methods, virus purification with Vivapure LentiSELECT is fast and simple, without the requirement of expensive equipment like an ultracentrifuge. Additionally, this chromatographic procedure leads to pure virus samples in contrast to the crude ultracentrifuge pellet, for achieving higher reproducibility and increased gene transfer efficiency.
Registered office at: 41 Bowhill, Kettering, NorthantsNN16 8TT, UK
Registered in England: 6892930
Tel: 01536 523604 Fax: 01536 210330
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